Unraveling Chemokine and Chemokine Receptor Expression Patterns Using Genetically Engineered Mice
Over the past 25 years, genetically engineered mouse models have become an integ
Over the past 25 years, genetically engineered mouse models have become an integral and invaluable research tool to develop our understanding of mammalian physiology and pathology. This unit describes methods for generating transgenic mice, focusing on reporter animals relevant to chemokine receptor and ligand expression. Specifically, we describe the use of bacterial artificial chromosome (BAC) engineering and embryonic stem cell manipulation to generate “knock in” and transgenic mice.
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Recombinant Protein Expression in the Baculovirus-Infected Insect Cell System
Over the last two decades, the use of eukaryotic cells for e...
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7 Determining Dye-Induced DNA Mobility Shifts for DNA Sequencing Fragments by Capillary Electrophore
Over the past decade, fluorescent end-labeling of DNA fragme...