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Unraveling Chemokine and Chemokine Receptor Expression Patterns Using Genetically Engineered Mice

2025-03-25 生物化学 3 加入收藏
Over the past 25 years, genetically engineered mouse models have become an integ

Over the past 25 years, genetically engineered mouse models have become an integral and invaluable research tool to develop our understanding of mammalian physiology and pathology. This unit describes methods for generating transgenic mice, focusing on reporter animals relevant to chemokine receptor and ligand expression. Specifically, we describe the use of bacterial artificial chromosome (BAC) engineering and embryonic stem cell manipulation to generate “knock in” and transgenic mice.

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