Monitoring of Phospholipase A2 Activation in Cultured Cells Using Tritiated Arachidonic Acid

Phospholipase A2 (PLA2 , EC 3.1.1.4, phosphatide sn -2 acyl hydrolase) is a ubiquitous enzyme that plays a role in the response of a wide variety of cells to specific stimuli. PLA2 is an important component of the cellular machinery that is activated by inflammatory stimuli and it maintains cell homeostasis by membrane remodeling. The role of PLA2 in the production of proinflammatory lipid mediators makes this enzyme an interesting therapeutic target for the treatment of inflammatory disorders (1 ).