Baculovirus Vectors
Recombmant baculovinrus expression systems are now firmly established as one of the tools for producing foreign proteins in high yield wlthin a eukaryotlc environment The evolution of both single- and multiple-transfer vectors and different forms of baculovlral DNA has greatly streainlined the lsolatlon and purlficatlon of a recoinblnant virus carrying the foreign gene of interest. This chapter summarize the wealth of transfer vectors and baculovnus DNAs available to the researcher to generate both single- and multiple-expressing recombinant baculovnuses and highlights the specific areas in which we feel that recombinant protein expressed via the baculovnus system has enabled siginficant advances to be inade
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Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria
Recombineering refers to a strategy for engineering DNA sequ...
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Determination of Monosaccharides Derivatized with 2-Aminobenzoic Acid by Capillary Electrophoresis
Reducing monosaccharides were derivatized with 2-aminobenzoi...