Cryopreservation of Shoot Tips and Meristems
Shoot-tip meristem cryopreservation methodologies are reported for the complementary cryoprotective strategies of vitrification and equilibrium freezing using traditional controlled-rate freezing and chemical additive cryoprotection. Pregrowth, pretreatment, and cold acclimation approaches for the improvement of tolerance to liquid nitrogen are also presented. The chapter concludes by reporting an analytical protocol that profiles volatile hydrocarbon stress markers (for ethylene, hydroxyl radicals, and lipid peroxidation products) during cryopreservation. This method uses noninvasive headspace sampling and gas chromatography, and it is widely applicable across cryogenic systems.
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Oscillating Probe for Dual Detection of 5'PO4 and 5'OH DNA Breaks in Tissue Sections
Several types of DNA cuts are used as markers of apoptosis f...
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Detecting Ordered Domain Formation (Lipid Rafts) in Model Membranes Using Tempo
Short-range fluorescence quenching has proven to be an effec...