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Preparation and Assay of Recombinant Serine Racemase

2025-03-27 生物化学 加入收藏
Serine racemase is a glial and neuronal enzyme that reversibly converts l -serin

Serine racemase is a glial and neuronal enzyme that reversibly converts l -serine to d -serine, an endogenous co-agonist of N -methyl-d -aspartate receptor type glutamate receptors (NMDARs). Here we present methods to recombinantly express and purify serine racemase in bacteria and two complementary ways to determine d -serine levels in unknown samples. Furthermore, a detailed protocol of serine racemase activity assays is described that can be used to screen for activators and inhibitors in vitro.

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