Nonradioactive Northern and Southern Analyses From Plant Samples
Several specific problems are encountered when nonradioactive detection methods
Several specific problems are encountered when nonradioactive detection methods are used in conjunction with plant nucleic acids. In this chapter, we describe protocols for the isolation of DNA and RNA from plant leaves and the preparation of probe molecules by either PCR or in vitro transcription with different haptens. Furthermore, standard conditions and possible modifications for hybridization and detection of probes are given.
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Use of three-dimensional collagen gels to study mechanotransduction in t47d breast epithelial cells
Several pathological and disease conditions can alter the me...
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Oscillating Probe for Dual Detection of 5'PO4 and 5'OH DNA Breaks in Tissue Sections
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