Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 生物化学

生物化学

Preparation, Restriction, and Hybridization Analysis of Mammalian Genomic DNA for Pulsed-Field Gel E

2025-03-27 生物化学 加入收藏
The aim of this chapter is to provide a very practical set of instructions that

The aim of this chapter is to provide a very practical set of instructions that will enable workers with some experience in standard DNA preparation and electrophoresis techniques to prepare and analyze mammalian DNA by pulsed-field gel electrophoresis (PFGE; see also refs. 1 and 2 for additional PFGE protocols). Detailed methods are provided that describe: sources of mammalian DNA, preparation of DNA, restriction enzyme digests, standard PFGE running conditions, preparation of size markers, and preparation, hybridization, and analysis of blots. Notes about potential pitfalls accompany each section together with a trouble-shooting guide in Section 5 .

文章底部广告位

文章评论

加载中~