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Multistep Enzyme Catalyzed Reactions for Unnatural Amino Acids

2025-03-29 生物化学 加入收藏
The use of unnatural amino acids, particularly synthetic α-amino acids, for mode

The use of unnatural amino acids, particularly synthetic α-amino acids, for modern drug discovery research requires the availability of enantiomerically pure isomers. Starting from a racemate, one single enantiomer can be obtained using a deracemization process. The two more common strategies of deracemization are those obtained by stereoinversion and by dynamic kinetic resolution. Both techniques will be here described using as a substrate the d ,l -3-(2-naphthyl)-alanine, a non-natural amino acid: the first one employing a multi-enzymatic redox system, the second one combining an hydrolytic enzyme together with a base-catalyzed substrate racemization. In both cases, the final product, l -3-(2-naphthyl)alanine, is recovered with good yield and excellent enantiomeric excess.

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