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Measurement of PTEN Activity in vivo by Imaging Phosphorylated Akt

2025-03-29 生物化学 加入收藏
This chapter describes an indirect approach to measure PTEN’s lipid phosphatase

This chapter describes an indirect approach to measure PTEN’s lipid phosphatase activity in vivo . PTEN counteracts phosphatidylinositol 3-kinase action in dephosphorylating 3-phosphorylated phosphoinositides. Therefore, PtdIns(3,4,5)P3 -dependent activation and phosphorylation of the survival kinase Akt can be used as readout for cellular PTEN activity. Here we have outlined a detailed procedure employing a phosphoserine-specific anti-Akt antibody to examine the content of phosphorylated Akt by immunofluorescence and its dependence on PTEN activity.

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