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Linkers, Resins, and General Procedures for Solid-Phase Peptide Synthesis

2025-03-29 生物化学 加入收藏
This chapter describes the basic protocols for solid-phase peptide synthesis usi

This chapter describes the basic protocols for solid-phase peptide synthesis using the Fmoc group as the N α -protecting group (Fmoc-SPPS). The chapter introduces resins and their handling, choice of linkers, and the most common methods for peptide chain assembly. The proper choice of resins and linkers for solid-phase synthesis is a key parameter for successful peptide synthesis. This chapter provides an overview of the most common and useful resins and linkers for the synthesis of peptides with C -terminal amides, carboxylic acids, and more. The chapter finishes with robust protocols for general solid-phase peptide synthesis, i.e., the standard operations.

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