Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 生物化学

生物化学

Subtilisin-Catalyzed Glycopeptide Condensation

2025-03-31 生物化学 加入收藏
This method describes the use of subtilisin-catalyzed peptide condensation to fo

This method describes the use of subtilisin-catalyzed peptide condensation to form a 15-residue glycopeptide from two smaller synthetic peptides. A 12-residue peptide ester is synthesized by solid-phase peptide synthesis using a PAM-modified Rink amide resin that allows the formation of a peptide ester suitable for subtilisin ligation. The 12-residue acyl donor peptide ester is then ligated to a 3-residue acyl acceptor glycopeptide amide using subtilisin (EC 3.4.21.62) in a buffered mixture of water and DMF (1:9).

文章底部广告位

文章评论

加载中~