An antibody-lectin sandwich assay for quantifying protein glycoforms
We describe an antibody-lectin sandwich assay for quantitation of glycoforms of
We describe an antibody-lectin sandwich assay for quantitation of glycoforms of proteins. The assay uses deglycosylated IgG antibody immobilized on a microtiter plate to capture the protein of interest from the sample. The particular glycoform is then identified by reaction with biotin-labeled lectin, which is measured using streptavidin/alkaline phosphatase. The assay can be adapted to quantitate any protein’s glycoforms by simply substituting the antibody and lectin with specific alternatives,
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Quantum Dot Hybrid Gel Blotting: A Technique for Identifying Quantum Dot-Protein/Protein-Protein Int
We describe an alternative to the molecular biology techniqu...
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An improved method for constructing and selectively silanizing double-barreled, neutral liquid-carri
We describe an improved, efficient and reliable method for t...