绿色荧光蛋白的应用

1 、骨架和细胞分裂

Kevin Sullivan's 实验室 酵母菌内 SPB 和微管动力学 酵母菌中肌动蛋白的动力 果蝇中 MEI-S332 蛋白 果蝇有丝分裂和 mRNA 运输 网丙菌属细胞骨架 RNA 剪切因子的核内运输 网丙菌属的趋化作用 网丙菌属中细胞骨架动力和细胞运动 核动力 网丙菌属中细胞动力 细胞骨架动力和胞内运输

2、细胞器动力学和泡囊运输

用 GFP 显示小囊运输 用 GFP 观察 TGN 运输 细胞骨架动力学和胞内运输

3 、 发育生物学

用 GFP 观察线虫的神经发育 分析果蝇神经发育的不对称性细胞分裂 线虫 Lin-14 Fischbach lab 用 GFP 观察网丙菌属的形态发生学 GFP 在小鼠发育中的标记方法

4 、 神经生物学

神经极性发育 tracking intracellular transport of peptide neurotransmitters using GFP Allen Lab Enhancer trapping using tau-GFP as reporter localized to axons

5、其他应用

Cell surface organization in Natural Killer cells Dan Davis localization of calmodulin in S. pombe with GFP GFP-plakoglobin and expression plasmids Klymkowsky lab Molecular Motion Laboratory Yale University Measuring diacylglycerol in vivo with a GFP-PKC chimera Tobias Meyer Bentley Lab using GFP for on-line bioprocess monitoring & control Tracking viral proteins with GFP fusions

6、GFP vectors and technology

Clontech Inc. Supplier of constructs for making GFP fusions DeltaVision 3D microscopy platform optimized for imaging GFP in living cells in real time GFP Expression Truly Amazing Web Site about GFP technology Universal Imaging Corp. makers of the MetaGFP image processing platform Quantum Biotechnologies Inc Suppliers of GFP and BFP constructs GFP in Arabidopsis BabCo/Covance antibody to GFP Lightools GFP plate illumination systems GFP in Chlamydomonas

7 、 Other Interesting GFP Links  

GFP Resources for Teachers Picture of Aequoria victoria, source of GFP Structure of green fluorescent protein Full text of Yang et al Nature Structural Bio paper Table of GFP mutant forms Optics in Cell Biology