Expression of Eukaryotic Cytochromes P450 in E. coli

Although much has been learned from the study of relatively abundant cytochromes P450 (e.g., P450cam from Pseudomonas putida cytosol, P4501A2 and P4502B4 from rabbit liver microsomes, and P45Oscc from bovine adrenal cortex mitochondria), the development of cytochrome P450 heterologous expression systems (l – 3 ), especially in Escherzchza coli (4 ), has provided an opportunity to address questions relating to the structure/function of less abundance dant forms of cytochrome P450 whose cDNAs (CYP cDNAs) have been isolated by recombinant DNA technologies. Among the advances achieved