果蝇头部收集--DROSOPHILA HEAD COLLECTION

DROSOPHILA HEAD COLLECTION(Baker Lab)

Equipment

flies frozen in 250 ml centrifuge bottles sieves: 106, 355, 600 and 850 mm paintbrush collection tubes funnel dry ice jacket, hat and gloves!

Preparation

1. Freeze flies in 250 ml centrifuge bottle(s) @ -80oC („30'). 2. Cool sieves @ -20oC for „ 20' prior to use.

Procedure

1. Arrange sieves as follows (top to bottom): parts retained 850 mm bodies 600 small bodies + some heads 355 heads 106 fly dust, legs

2. Shake flies with moderate strength 4-6X for 3" each time.

3. Pour flies onto top sieve.

4. Brush gently so as not to jam flies into mesh openings. Females tend to be a little larger than males; you may want to pass the 600mm material back over the 850 mm sieve 2- 4X.

5. Collect heads into tared tubes. Keep frozen until used. Obtain a weight (?).

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