RNA purification --- hot phenol protocol

Hot phenol may also be used to remove DNA.

1. Add equal volume of TE-saturated phenol to RNA solution. Mix.

2. Heat to 70 ℃ for 5 minutes.

3. Centrifuge at top speed for 10 minutes at room temperature.

4. Transfer the aqueous phase to a fresh tube.

5. Proceed to extract with cold phenol:chloroform:isoamyl alcohol.