常用的细胞因子引物表
细胞因子引物序列产物(bp)IL-1a5’ ATGGCCAAAGTTCGAGACATG 3’ CTACGCCTGGTTTTCCAGTATCTGAAAGTCAGT
| 细胞因子 | 引物序列 | 产物(bp) |
| IL-1a | 5’ ATGGCCAAAGTTCGAGACATG 3’ CTACGCCTGGTTTTCCAGTATCTGAAAGTCAGT | 816 |
| IL-1b | 5’ ATGGCAGAAGTACCTAAGCTC 3’ TTAGGAAGACACAAATTGCATGGTGAACTCAGT | 810 |
| IL-2 | 5’ ATGTACAGGATGCAACTCCTG 3’ TCAAGTCAGTGTTGAGATGATGCTTTGACAAAA | 462 |
| 5’ TAACCTCAACTCCTGCCACAATGTACA 3’ ATGTTGTTTCAGATCCCTTTAGTTCCAGA | 386 | |
| IL-3 | 5’ ATGAGCCGCCTGCCCGTCCTG 3’ AAGATCGCGAGGCTCAAGTCGTCTGTTG | 459 |
| IL-4 | 5’ ATGGGTCTCACCTCCCAACTG 3’ TCAGCTCGAACACTTTGAATATTTCTCTCTCAT | 462 |
| 5’ TCTCACCTCCCAACTGCTTC 3’ AGAGGTTCCTGTCGAGCCGTTTCA | 337 | |
| IL-5 | 5’ ATGAGGATGCTTCTGCATTTG 3’ TCAACTTTCTATTATCCACTCGGTGTTCATTAC | 414 |
| IL-6 | 5’ ATGAACTCCTTCTCCACAAGC 3’ CTACATTTGCCGAAGAGCCCTCAGGCTGGACTG | 639 |
| IL-7 | 5’ ATGTTCCATGTTTCTTTTAGG 3’ AGCTTTTCTTTAGTGCCCATCAAAATTTTATTCCAACA | 707 |
| IL-8 | 5’ ATGACTTCCAAGCTGGCCGTG 3’ TTATGAATTCTCAGCCCTCTTCAAAAACTTCTC | 302 |
| IL-9 | 5’ CATCTTTTCTGAGGAATG(AG)TATTTC 3’ AAAACCCTGACATCAAACTGATACC | 110 |
| IL-10 | 5’ AGGGCACCCAGTCTGAGAACA 3’ CGGCCTTGCTCTTGTTTTCAC | 351 |
| IFN-g | 5’ ATGAAATATACAAGTTATATG 3’ TTACTGGGATGCTCTTCGACCTCGAAACAGCAT | 501 |
| 5’ TTTTCAGCTCTGCATCGTTTTGGGT 3’ CCTTGAAACAGCATCTGACTCCTT | 456 | |
| IFN-a | 5’ ATGAGCACTGAAAGCATGATC 3’ TCACAGGGCAATGATCCCAAAGTAGACCTGCCC | 702 |
| IFN-b | 5’ ATGACACCACCTGAACGTCTCTTC 3’ CTACAGAGCGAAGGCTCCAAAGAAGACAGTACT | 608 |
| TNF-a | 5’ ATGAGCACTGAAAGCATGATC 3’ TCACAGGGCAATGATCCCAAAGTAGACCTGCCC | 702 |
| 5’ AGGCGGTGCTTGTTCCTCA 3’ GTTCGAGAAGATGATCTGACTGCC | ||
| TNF-b | 5’ ATGACACCACCTGAACGTCTCTTC 3’ CTACAGAGCGAAGGCTCCAAAGAAGACAGTACT | 608 |
| TGF-b1 | 5’ AACATGATCGTGCGCTCTGCAAGTGCAGC 3’ AAGGAATAGTGCAGACAGGCAGGA | 200 |
| b-Actin | 5’ GTGGGGCGCCCCAGGCACCA 3’ CTCCTTAATGTCACGCACGATTTC | 548 |
| 5’ ATTGCCGACAGGATGCAGAA 3’ GCTGATCCACATCTGCTGGA | 150 |
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