PriCells: Isolation, culture, and purification of Human Pulmonary Arterial Smooth Muscle Cells

PriCells: Isolation, culture, and purification of Human Pulmonary Arterial Smooth Muscle Cells (HPASMCs)

1. HPASMCs were isolated from lungs resected from tissue of normal human or adult patients with malignant tumors.2. The arteries were separated from their adventitia and endothelium and then minced into 1- to 3-mm2 pieces with sterile scalpel blades. 3. The tissues were mounted in tissue culture wells. 4. The HPASMCs pellets were resuspended in Pricells Culture System and cultivated on 6-well plates pre-coated with gelatin (0.2%) for several days until subconfluent. 5. Purification: The HPASMCs were isolated using magnetic beads (Dynal) according to the instructions of the manufacturer. 6. The medium was replaced every 2 to 3 days, and the subcultures were obtained by trypsin/EDTA treatment of confluent monolayers at a splitting ratio of 1:3.7. Identification: Cells were identified with PriCells Identification System.

PriCells: Primary cell products:

1. Primary cell extract2. Primary cell RNA3. Primary cell DNA4. Primary cell base medium5. Primary cell growth supplement6. Primary cell isolation kit7. Primary cell identification kit8. Primary cell transfection kit