Apoptosis: A Laboratory Manual of Experimental Methods

HE CELL 1. Morphological aspects of apoptosis Walter Malorni, Stefano Fais & Carla Fiorentini 2. Cell cycle Miriam Capri & Daniela Barbieri

THE NUCLEUS 3. Fluorescence microscopy analysis of nuclear alterations during apoptosis Ildo Nicoletti & Roberta Mannucci 4. Qualitative analysis of DNA fragmentation by agarose gel electrophoresis Paola Bossu’ 5. Fluorochrome labelling of DNA strand breaks by terminal deoxynucleotidyl transferase (TdT-assay) and analysis by flow cytometry Ildo Nicoletti & Roberta Mannucci 6. A whole blood method for apoptosis determination Claudio Ortolani, Gabriella Scuccato, Roberto Cibin, Alessandro De Toffoli, Paola Arslan 7. Multiparametric analysis by flow cytometry Sandra Nuti 8. Detection of apoptosis in situ with immunocytochemistry and TUNEL Daniela Barbieri & Enzo Ottaviani 9. Quantitation of DNA fragmentation with diphenylamine Diana Boraschi & Giovanni Maurizi 10. APOPTOSIS vs. NECROSIS Marco Vitale, Giorgio Zauli & Elisabetta Falcieri

THE CYTOPLASM 11. Caspases Ann Zeuner, Maria Rita Rippo, Ruggero De Maria & Roberto Testi 12. Sphingomyelinases Luisa Di Marzio & Maria Grazia Cifone 13. Tissue transglutaminase Maria Elisabetta Cosulich & Marina Fabbi 14. Analysis of mitochondrial membrane potential Andrea Cossarizza & Stefano Salvioli 15. Cytoskeleton Carla Fiorentini, Paola Matarrese, Alessia Fabbri & Walter Malorni

THE PLASMA MEMBRANE 16. ANNEXIN V Stefania Morrone 17. Differential light scattering and CD45 membrane fluorescence Maurizio Carbonari 18. Modification of antigen expression in apoptotic cells: a single laser methodology Filippo Reno’ & Stefano Papa