-
lncRNA 通过调控 CD56 参与自然杀伤细胞发育过程
自然杀伤(NK)细胞是一类先天性免疫淋巴细胞,与体外病毒感染和免疫调节有关。但是,lncRNA在NK细胞生物学过程中的作用还不是很清楚。研究人员借助于高通量ln
-
Gene Expression Profiling in Cancer Using cDNA Microarrays
The principle of cDNA microarray hybridization takes advantage of the property o
-
外源基因在真核细胞表达步骤
在真核细胞中表达蛋白的步骤比大肠杆菌复杂得多。首先要考虑选用什么载体。一般人们趋向于使用分泌表达载体,以便于蛋白的纯化。但有的蛋白并不适合于分泌表达,如一些胞质
-
sf9,空斑试验,滴度测定
【求助】sf9,空斑试验,滴度测定参与者:xiaoniziwendeng我用的是bac-to-bac系统表达的目的蛋白。加上荧光底物后,96孔板读数,野生病毒和
-
Lung Cancer and Exposure to Metals: The Epidemiological Evidence
Exposure to metallic compounds is ubiquitous, with its widespread use in industr
-
Epigenetic Markers of Early Tumor Development
Cancer patients’ outcome and survival depends on the early diagnosis of malignan
-
Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site Anal
The completion of the human genome sequence and availability of cDNA microarray
-
Collagen Invasion Assay
Invasion occurs when invasion promoter molecules outbalance the function of inva
-
用于细胞分析的机器视觉系统简介
1996年,美国盐湖域的阿鲁普实验室遇到一个问题。该实验室从事帕帕尼科拉乌涂片的筛分工作以便找出宫颈癌症兆。当涂片上有少量异常细胞存在而又未被探测出来时,他们会
-
Use of Splicing Reporter Minigene Assay to Evaluate the Effect on Splicing of Unclassified Genetic V
The interpretation of the numerous sequence variants of unknown biological and c
-
G-Rich Oligonucleotides for Cancer Treatment
Oligonucleotides with guanosine-rich (G-rich) sequences often have unusual physi
-
Construction and Characterization of Minibodies for Imaging and Therapy of Colorectal Carcinomas
Engineering of antibodies specific for tumor-associated antigens provides great
-
软琼脂克隆形成实验
原理:细胞接种存活率只表示接种细胞后贴壁的细胞数,但贴壁后的细胞不一定每个都能增殖和形成克隆。而形成克隆的细胞必为贴壁和有增殖活力的细胞。克隆形成率反映细胞群体
-
真核生物翻译的调控
原核生物基因表达的调控主要在转录水平上进行,而真核生物由于RNA较为稳定,所以除了存在转录水平的调控以外,在翻译水平上也进行各种形式的调控。在蛋白质生物合成的起
-
d-Glucaric Acid as a Prospective Tumor Marker
d -Glucaric acid (GA) 1s a natural, apparently nontoxic compound produced in sma