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A Cell-Free System for Reconstitution of Transport Between Prevacuolar Compartments and Vacuoles in
Genetic approaches have revealed more than 50 genes involved in the delivery of
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Chiral Separations by Simulated Moving Bed Method Using Polysaccharide-Based Chiral Stationary Phase
Generally, in using chromatography as an industrial-scale production process, ba
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In Vivo Analysis of Protein Translocation to the Escherichia coli Periplasm
General protein export requires the cooperation of two elements, the Sec translo
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Detecting ProteinSmall Molecule Interactions Using Fluorous Small-Molecule Microarrays
General binding assays involving microarrays of small molecules can be used to i
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Silver Staining Protocol for Acrylamide Gels
Gel MixFor 1 litre solutionUrea (AG)453 gms10X TBE50 ml40%(19:1 ratio) Acrylamid
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Elastic Bag Model of One-Dimensional Pulsed-Field Gel Electrophoresis (ODPFGE)
Gel electrophoresis is one of the most common techniques used in molecular biolo
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Use of Gas-Phase Fractionation to Increase Protein Identifications: Application to the Peroxisome
Gas-phase fractionation (GPF), defined as iterative mass spectrometric interroga
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盖阿假说(Gaia hypothesis)
Gaia一词,直译大地女神,故盖阿假说也称大地女神假说。1965年,英国大气化学家拉夫洛克(J.E.Lovelock)提出。他认为,地球表面的温度和化学组成是
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monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOC
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Measurement of Nitric Oxide-Related Enzymes in the Brain by In Situ Hybridization
Functional genomic increasingly requires the cell-specific localization of gene
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Cryopreservation of Rat Hepatocytes in a Three-Dimensional Culture Configuration Using a Controlled-
From engineered tissues to transfected cell lines, the long term storage of livi
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Recent advances in freeze-fracture electron microscop: the replica immunolabeling technique
Freeze-fracture electron microscopy is a technique for examining the ultrastruct
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Lipoic Acid as an Antioxidant: The Role of Dihydrolipoamide Dehydrogenase
Free lipoic acid is present in biological systems at extremely low levels. The b
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Fragment-Based Drug Design
Fragment-based drug design (FBDD), which is comprised of both fragment screening
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An Enhanced Antigen-Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-
Formalin is the most commonly used fixative for light microscopy because of its