Small-Scale Plasmid DNA Preparation
For the initial characterization of a recombinant plasmid, it is necessary to de
For the initial characterization of a recombinant plasmid, it is necessary to determine the size of the plasmid or, preferably, the size and characteristics of the insert itself. A method is therefore required for the simultaneous preparation, from a number of isolates, of plasmid DNA in a state sufficiently pure for restriction enzyme digestion. The requirements of such a procedure are:
| (i) | A simple method for rapid lysis of the bacterial cells. |
| (ii) | Separation of plasmid from chromosomal DNA. |
| (iii) | Removal of proteins and of other components of the cells that might interfere with restriction enzyme treatment. |
| (iv) | Removal of detergents, salts, etc. used in the process. |
相关文章
