Login
欢迎浏览恩派尔生物资料网
我要投稿 请登录 免费注册 安全退出

您现在的位置是: 首页 > 实验方法 > 生物化学

生物化学

A Homogeneous Immunoassay of Thyroxine Based on Microchip Electrophoresis and Chemiluminescence Dete

2025-05-06 生物化学 加入收藏
A homogeneous chemiluminescent immunoassay of thyroxine (T4) present in serum sa

A homogeneous chemiluminescent immunoassay of thyroxine (T4) present in serum samples is described. The proposed method deployed the competitive immunoreaction of T4 and horseradish peroxidase (HRP)-labeled T4 (HRP–T4) with anti-T4 mouse monoclonal antibody (Ab). HRP–T4 and the HRP–T4–Ab complex were separated and quantified by using microchip electrophoresis (MCE) with chemiluminescence (CL) detection. The MCE separation was accomplished within 60 s. Highly sensitive CL detection was achieved by means of HPR-catalyzed luminol-H2O2 reaction. The linear range for T4 was 5–250 nM with a detection limit of 2.2 nM (S/N = 3).

文章底部广告位

文章评论

加载中~