组织学――显微解剖

Laser Capture Microdissection (LCM)

• Introduction to LCM  (BJMU)   
• Preparation, LCM and RNA/DNA extraction of Frozen Tissue Sections (NIH Laser Capture Microdissection Core Lab)
• Preparation and LCM of Paraffin Embedded Tissue Sections (NIH Laser Capture Microdissection Core Lab)   
• DNA Extraction Protocol of LCM tissue and PCR for DNA analysis (NIH Laser Capture Microdissection Core Lab)   
• RNA Extraction Protocol of LCM tissue and RT-PCR for mRNA analysis (NIH Laser Capture Microdissection Core Lab)    
• Paraffin-Embedded Tissue for LCM (Arcturus) Paraffin embedding is a process in which fixed (formalin or other fixative) is infiltrated and then blocked out in paraffin to stabilize it for long-term storage and easy sectioning. ..   
• H & E Staining Protocol for LCM (Arcturus)   
• De-Paraffinization of Tissue Sections (Arcturus) Remove paraffin from section for tissue preparation such as nucleic acid extraction.    
• DNA Extraction From Paraffin Section (Arcturus) This extraction method has been used for measuring loss of heterozygosity (LOH), dideoxy fingerprinting (DDF), clonality analysis (chromosome X inactivation) and direct sequencing of PCR* products for single base mutational analysis. Extractions are typically performed on 500-1000 captured cells.   
• RNA Extraction from Paraffin Section (NIH Protocol) (Arcturus) A Protocol for extracting RNA from paraffin embedded tissue.   
• cDNA Library Construction

  Detailed protocol for cDNA library construction from microdissected material.