抗体纯化

Antibody Purificatioin

• Purification of IgG Using Protein A- or Protein G-Agarose (KPL) 
• Purifying Antibodies (Perkin-Elmer)

Precipitation MethodsProtein A or Protein G Columns or BeadsAntigen Columns

・         Affinity Purification of Antibodies from Crude Serum (PMCI)

・         Affinity Purification of Antibody (Walter Steffen)

• • Coupling procedure
  • Affinity purification
  • Absorption of antibodies to acetone powder
  • Production of acetone powder
  • Extraction of antibodies from egg yolk
  • Purification and cleavage of murine monoclonal antibodies
  • IgG purification with protein G
  • Digest of IgG with papain  

・         Affinity Purification of Antibodies (Vesicle Trafficking)

・         Affinity Purification of Antibodies (XMMR)protocol in great detail, including column preparation, wash and more...

・         Protein G Purification of Antibodies (Contributed by Nanci Donacki) 

・         Protein A Purification of Antibody (Contributed by Nanci Donacki) 

• Coupling Antibodies to Protein A/G (Breeden Lab)  
• Coupling Peptides to Resin for Affinity Purification (Mitshison Lab)  
• Purification of Anti Peptide Antibody (Mitshison Lab)     
• Antibody Purification (Mike A. Dyer) This protocol includes an ammonium sulfate cut, affigel blue chromatography and affinity chromatography.

Column Preparation

• Making Affinity Columns (XMMR)

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