单克隆抗体

Tips and hints for the storage of antibodies (Synaptic Systems)

・         Purification of IgG Using Protein A- or Protein G-Agarose (KPL)

・         Production  of Monoclonal Antibody (Chisholm Lab)Protocol for making monoclonal antibodies. All steps of the entail procedure are described in great details

・         Production of Monoclonal Antibody in Mice  (Walter Steffen)Whole procedures for monoclonal antibody production

・         Preparation of Monoclonal Antibody (PMCI)Protocol for making hybridoma and antibody

• Cell Fusion and Selection (PMCI)
• Fusion of Mouse, Rat, or Hamster Cell (Contributed by Nanci Donacki)  
• Fusion and Cloning (Contributed by Nanci Donacki)Procedure for establishing hybridoma in one step  
• Cloning by Limiting Dilution (Contributed by Nanci Donacki)  

・         Affinity Purification of Monoclonal Antibodies From Tissue (PMCI)  

・         Expanding Hybridoma Clones (PMCI)   

・         Protein G Purification of Antibody(Contributed by Nanci Donacki)  

・         Protein A Purification of Antibody (Contributed by Nanci Donacki)