HLA-DR基因中等分辨率分型序列特异性引物
HLA-DR 基因中等分辨率分型序列特异性引物 5’-引物序列(5’ � 3’)3’-引物序列(5’ � 3’)PCR产物长度DRB1基因扩增特异性5’01T
HLA-DR 基因中等分辨率分型序列特异性引物
| 5’-引物 | 序列(5’ � 3’) | 3’-引物 | 序列(5’ � 3’) | PCR产物长度 | DRB1基因扩增特异性 |
| 5’01 | TTGTGGCAGCTTAAGTTTGAAT | 3’047 | CTGCACTGTGAAGCTCGCAC | 255bp | 0101-0102 |
| 3’048 | CTGCACACTGTGAAGCTCTCCA | 255bp | |||
| 5’01 | TTGTGGCAGCTTAAGTTTGAAT | 3’10 | CCCGCTCGTCTTCCAGGAT | 130bp | 0103 |
| 5’02 | tcctgtggcagcctaagag | 3’01 | CCGCGCCTGCTCCAGGATT | 197bp | 1501,1502 |
| 5’02 | tcctgtggcagcctaagag | 3’03 | AGGTGTCCACCGCGGCG | 213bp | 1601,1602 |
| 5’03 | TACTTCCATAACCAGGAGGAGA | 3’03 | TGCAGTAGTTGTCCACCCG | 151bp | 0301,0302 |
| 5’06 | GACGGAGCCGGTGCGTA | 3’48 | CTGCACACTGTGAAGCTCTCCA | 217bp | 0301 |
| 5’03 | TACTTCCATAACCAGGAGGAGA | 3’47 | CTGCACTGTGAAGCTCGCAC | 189bp | 0302,1302,1305 |
| 1402,1403,1409 | |||||
| 5’04 | GTTTCTTGGAGCAGGTTAAACA | 3’47 | CTGCACTGTGAAGCTCGCAC | 260bp | 0401 -0411 |
| 3’048 | CTGCACACTGTGAAGCTCTCCA | 260bp | |||
| 5’07 | CCTGTGGCAGGGTAAGTATA | 3’079 | CCCGTAGTTGTGTCTCGACAC | 232bp | 0701,0702 |
| 5’08 | ACTACTCTACGGGTGAGTGTT | 3’05 | CTGCAGTAGGTGTCCACCAG | 214bp | 0801-0806 |
| 5’09 | gtttcttgaagcaggataagttt | 3’079 | CCCGTAGTTGTGTCTCGACAC | 236bp | 0901 |
| 5’10 | gttcttggagtactctacgtc | 3’047 | CTGCACTGTGAAGCTCGCAC | 204bp | 1001 |
| 5’05 | gttCTTGGAGTACTCTACGTC | 3’06 | CTGGCTGTTCCAGTACTCCT | 176bp | 1101 - 1105 |
| 5’08 | ACTACTCTACGGGTGAGTGTT | 3’08C | TCTGTGAAGCTCTCCACAG | 248bp | 1201,1202 |
| 5’03 | TACTTCCATAACCAGGAGGAGA | 3’10 | CCCGCTCGTCTTCCAGGAT | 130bp | 1301,1302 |
| 5’05 | gttCTTGGAGTACTCTACGTC | 3’11 | TCTGCAATAGGTGTCCACCT | 224bp | 1401,1404,1405 |
| 5’08 | ACTACTCTACGGGTGAGTGTT | 1407,1408 | |||
| 5’03 | TACTTCCATAACCAGGAGGAGA | 3’12 | TCCACCGCGGCCCTCC | 140bp | 1402,1404,1409 |
| 1305,1306 | |||||
| 5’04 | GTTTCTTGGAGCAGGTTAAACA | 3’19 | ctgttccagtctccgcag | 188bp | 1410 |
| 5’52.1 | TTTCTTGGAGCTGCGTAAGTC | 3’13 | CTGTTCCAGGATCGGGCGA | 171bp | DRB3*0101 - 0301 |
| 5’52.2 | GTTTCTTGGAGCTGCTTAAG | 3’14 | GCTGTTCCAGTACTCGGCAT | 173bp | |
| 5’53 | GAGCGAGTGTGGACCTGA | 3’048 | CTGCACACTGTGAAGCTCTCCA | 213bp | DRB4*0101 |
| 5’51 | GTTTCTTGCAGCCAGGATAAGTA | 3’01 | CCGCGCCTGCTCCAGGATT | 198bp | DRB5*0101 �0 202 |
| 3’16 | CCGCGGCGGCCTGTCT | 207bp |
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