Determination of Cell Tropism of HIV-1

With the discovery that changes in the biological properties of HIV-1 correlate with the progression to disease, it became more and more important to develop assays to distinguish between the viral phenotypes. In this chapter, it is described how the biological phenotype of HIV-1 with regard to cellular tropism can be determined on primary monocyte-derived macrophages, established T-cell lines MT2 , SupT1 , and H9, and promonocytic cell lines U937, HL-60, and THP-1.