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Staining Methods for cell death Z. Xia 10/2/95
The simplest way: trypan blue. Dead cells stain blue Non-fixed cells: FDA(fluore
2025-03-06 22 -
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
Description This protocol is for use with the D cyclins and employs 488 nm argon
2025-03-06 20 -
Localization of the myc-Tagged 5HT5A Receptor by Immunogold Staining of Ultrathin Sections
Immunohistochemistry is a powerful technique to localize proteins in tissues and
2025-03-06 22 -
Online Tools for Predicting Integral Membrane Proteins
We identify and describe a set of tools readily available for integral membrane
2025-03-06 21 -
核酸酶保护分析简介
相关专题 核糖核酸酶保护实验核酸酶保护法是一种用于生物化学和遗传学识别个人在异构RNA的提取样品RNA分子细胞实验室技术。 该技术可以识别一个或多个已知序列的R
2025-03-06 19 -
Reconstitution System Based on Cytosol-Depleted Cells to Study the Regulation of Phospholipase D
Phospholipase D (PLD) hydrolyzes phosphatidylcholine to produce the membraneasso
2025-03-06 17 -
转基因――材料和试剂
Media and Solutions Required for Routine ES Cell Culture: Version 1 (PMCI) Medi
2025-03-06 19 -
细胞凋亡物质释放的反馈调节
相关专题 线粒体 跨膜通透性通道的形成以及线粒体溶胀解体导致大量细胞色素c和Apaf-1、AIF等促凋亡因子的释放,这些因子会激活相应的caspase,cas
2025-03-06 21 -
RNA-Dependent RNA Switches in Bacteria
The versatility of RNA as a regulatory molecule has become increasingly apparent
2025-03-06 17 -
研究细胞凋亡的意义
相关专题 细胞的作用雷蒙德.戴维斯( R aymond Davis Jr),美国,美国宾州大学物理天文系1914年生于美国。小柴昌俊( M asatoshi K
2025-03-06 21 -
用于融合试验的主要骨髓瘤细胞系
名 称 来 源 耐 受 药 物 Ig 链 H L P3/X63-Ag8(X63) BALB/C 骨髓瘤 MOPC-21 8- 氮鸟嘌呤 r1 K P
2025-03-06 22 -
贴壁细胞的消化方法介绍
相关专题 一、酶消化法。1、胰酶。这是用得最多的。一般浓度在0.25-0.5%。作用时间根据细胞种类、作用温度等因素而变化很大,从几分钟到几十分钟不等。0.2
2025-03-06 32 -
Quantitation of Murine and Human Hematopoietic Stem Cells by Limiting-Dilution Analysis in Competiti
In designing functional assays for the various classes of hematopoietic cells de
2025-03-06 28 -
叶绿体(chloroplast)
植物细胞所特有的能量转换细胞器, 其功能是进行光合作用,即利用光能同化二氧化碳和水, 生成糖, 同时产生分子氧。叶绿体除了双层膜结构外,还有类囊体, 它是叶绿体
2025-03-06 46 -
装配反应因子(assembly reaction factor, ARF)
一类与被膜小泡装配相关的蛋白因子。在COP被膜小泡装配中, ARF被认为是外被体外被的装配和去装配的信号。装配反应因子是一种单体GTPase,由一条多肽链组成,
2025-03-06 21