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  • 胞吐作用(exocytosis)

    胞吐作用(exocytosis)

    运输小泡通过与细胞质膜的融合将内容物释放到细胞外基质的过程称为胞吐作用, 膜融合是通过融合蛋白的帮助完成的。在组成型分泌活动中,胞吐作用是自发进行的,但是在调节

    2025-02-27 24
  • Xeno-Free Culture of Human Pluripotent Stem Cells

    Xeno-Free Culture of Human Pluripotent Stem Cells

    Stem cell culture systems that rely on undefined animal-derived components intro

    2025-02-27 24
  • 锌指结构基元(zinc finger motif)

    锌指结构基元(zinc finger motif)

    这类蛋白因子往往含有几个相同的指结构, 每一个指结构具有以下的保守序列: Cys-X2-4-Cys-Phe-X5-Leu-X2-His-X3-His。这段保守序

    2025-02-27 23
  • Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins

    Visualization of the Expression of Green Fluorescent Protein (GFP)-Linked Proteins

    One of the most remarkable and unexpected advances in cell biology in the 1990s

    2025-02-27 128
  • In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C

    In Vitro Assays for Cobblestone Area-Forming Cells, LTC-IC, and CFU-C

    Various assays exist that measure the function of hematopoietic stemcells (HSCs)

    2025-02-27 39
  • 人白细胞介素33(IL-33)酶联免疫分析(ELISA)

    人白细胞介素33(IL-33)酶联免疫分析(ELISA)

    人 白细胞介素33(IL-33) 酶联免疫 分析( ELISA )试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相

    2025-02-27 43
  • Engineering of a 3D Nanostructured Scaffold Made of Functionalized Self-Assembling Peptides and Enca

    Engineering of a 3D Nanostructured Scaffold Made of Functionalized Self-Assembling Peptides and Enca

    Three-dimensional (3D) in vitro models of cell culture aim to fill the gap betwe

    2025-02-27 37
  • Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR

    Analysis of Suppressor of Cytokine Signalling (SOCS) Gene Expression by Real-Time Quantitative PCR

    The Suppressor of Cytokine Signalling (SOCS) proteins are a family of negative r

    2025-02-27 42
  • 细胞培养常见问题解析

    细胞培养常见问题解析

    相关专题1、如何选用培养基?培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞,很可能在DMEM或M199中同样很容易生长。总之,首选MEM做粘附细胞培养

    2025-02-27 44
  • Freezing cells in liquid nitrogen

    Freezing cells in liquid nitrogen

    Take off Media Trypsinate with 1ml x2 Dulbecco A trypsin Add 7ml Media Pipette u

    2025-02-27 37
  • Analysis of Pyroptosis in Bacterial Infection

    Analysis of Pyroptosis in Bacterial Infection

    Eukaryotic cells undergo death by several different mechanisms: apoptosis, a cel

    2025-02-27 35
  • DNA导入哺乳动物细胞常见的方法

    DNA导入哺乳动物细胞常见的方法

    名称 机制 转染效率 用途 优缺点 影响因素 磷酸钙转染法 内吞作用 20%细胞被转染 瞬时表达 1、简单有效 2、适用于贴壁、非贴壁细胞   3、常作首选方法

    2025-02-27 82
  • Chlamydomonas reinhardtii: The Model of Choice to Study Mitochondria From Unicellular Photosynthetic

    Chlamydomonas reinhardtii: The Model of Choice to Study Mitochondria From Unicellular Photosynthetic

    Chlamydomonas reinhardtii is a model organism to study photosynthesis, cellular

    2025-02-27 25
  • Control of Vascular Tube Morphogenesis and Maturation in 3D Extracellular Matrices by Endothelial Ce

    Control of Vascular Tube Morphogenesis and Maturation in 3D Extracellular Matrices by Endothelial Ce

    An important advance using in vitro EC tube morphogenesis and maturation models

    2025-02-27 34
  • 内吞(endocytosis)

    内吞(endocytosis)

    大分子及颗粒物质进入细胞的方式。细胞膜对大分子物质是不通透的,细胞通过内吞摄入这类物质,首先,摄入物附着于细胞表面,被周围质膜逐渐包围,随之质膜内陷,最后分离下

    2025-02-27 59