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Staining Methods for cell
death Z. Xia 10/2/95 The simplest way: trypan blue. Dead cells stain blue Non-fi
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实验:用淋巴细胞分离液分离单个核细胞
相关专题 1)分离外周血中的单个核细胞(PBMC) 1.抽取正常人静脉血加到肝素抗凝管中,加等量含5~10IU/ml肝素的无血清缓冲液悬浮细胞。将细胞悬液小心
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细胞自发转化spontenous transformation
1.什么是细胞转化?何时会发生细胞转化? 细胞转化是细胞发生遗传性状改变的一种变化,它的基础是DNA或基因的突变。从属性上说,基因突变导致生物体发生的转化,有利
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支原体(mycoplasma)
又称霉形体,是最简单的原核细胞,支原体的大小介于细菌与病毒之间,直径为0.1~0.3 um, 约为细菌的十分之一, 能够通过滤菌器。支原体形态多变,有圆形、丝状
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Generation of Induced Pluripotent Stem Cell Lines from Human Fibroblasts via Retroviral Gene Transfe
This chapter describes a protocol for deriving induced pluripotent stem cells (i
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Modulation of the Host Skeletal Muscle Niche for Donor Satellite Cell Grafting
Skeletal muscle tissue has a remarkable capability of regenerating in pathologic
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Measuring the Absolute Abundance of the Smad Transcription Factors Using Quantitative Immunoblotting
In the age of systems biology, biologists seek to quantify the absolute number o
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Tissue Culture Media
We use two different kinds of media. Most cells are grown in DMEM. A few lymphoi
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液态镶嵌模型
各种物质特别是生物大分子在各种生物结构中的特殊有序排列,是各种生命现象得以实现的基础。70年代初期,singer和nicholson提出的液态镶嵌模型(flui
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Preparation of Segmented and Polarity Marked Microtubules
Preparation of Segmented and Polarity Marked Microtubules Segmented and polarity
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MAPK signaling pathway - Homo sapiens (human)
相关专题 MAPK信号通路专题MAPK signaling pathway - Homo sapiens (human):The mitogen-activat
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细胞坏死(necrosis)
细胞坏死是因病理而产生的被动死亡,如物理性或化学性的损害因子及缺氧与营养不良等均导致细胞坏死。坏死细胞的膜通透性增高,致使细胞肿胀,细胞器变形或肿大,早期核无明
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Basic Method for Direct Immunofluorescence Labeling
Basic Method for Direct Immunofluorescence LabelingBackgroundThis is the method
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原代软骨细胞分离培养的总结
以兔软骨细胞为例: ①一般根据实验要求,选取不同年龄组的兔子,实际上兔子的年龄越小越好,毕竟幼体组织的活力要高于成体组织的活力,耳静脉空气注射法处死后,无菌条件
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肿瘤细胞的培养
`7j5c Nt8l -o 肿瘤细胞培养 P.} 分析化学, 论坛, 化学分析, 仪器分析, 分析测试, 色谱, 电泳, 光谱#t$d%m"?4d 肿
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