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肺癌动物模型建立-焦油沥青 (GTP) 法构建肺癌动物模型
简介焦油沥青 (GTP) 法是通过向气管内注入焦油沥青，从而诱发肺癌。材料与仪器动物模型：体重 150- 200 g 大鼠（初断乳，饲养 4 周）器材：窥耳镜、
2024-12-24 22 -
Methods to Study the Biogenesis of Membrane Proteins in Yeast Mitochondria
The biogenesis of mitochondrial membrane proteins is an intricate process that r
2024-12-24 16 -
Methods for Assessing Autophagy and Autophagic Cell Death
Autophagic (or type 2) cell death is characterized by the massive accumulation o
2024-12-24 23 -
Cultivation of Normal Human Epidermal Melanocytes
An important approach in studies of normal, diseased, and malignant cells is the
2024-12-24 32 -
Isolation and Characterization of Human Embryonic Stem Cells
The derivation of human embryonic stem (hES) cells is a challenging procedure. T
2024-12-24 20 -
The Application of X-Ray Absorption Spectroscopy to Characterize Metal Centers in Proteins
The availability of synchrotron radiation has transformed the status of X-ray ab
2024-12-24 15 -
Drosophila Hemocyte Migration: An In Vivo Assay for Directional Cell Migration
This protocol describes an in vivo assay for random and directed hemocyte migrat
2024-12-24 15 -
Carbohydrate-Specific Adhesion of Intact Cells to Resolved Glycolipids on TLC Plates
T his procedure allows detection of specific cell adhesion to glycolipids resolv
2024-12-24 16 -
Use of Multiphoton Imaging for Studying Cell Migration in the Mouse
We describe a method for studying cell motility in the living mouse using multip
2024-12-24 19 -
Purification and Assay of Kinase-Active EGF Receptor From Mammalian Cells by Immunoaffinity Chromato
The epidermal growth factor (EGF) receptor possesses intrinsic protein-tyrosine
2024-12-24 16 -
程序性细胞死亡(programmed cell death,PCD)
又称为细胞凋亡(apoptosis)。程序性细胞死亡是指为维持内环境稳定，由基因控制的细胞自主的有序性的死亡，它涉及一系列基因的激活、表达以及调控等的作用，因而
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细胞培养常见问题及其解决方法[3]
相关专题1. 如何选用特殊细胞系培养基？ 培养某一类型细胞没有固定的培养条件。在MEM中培养的细胞，很可能在DMEM或M199中同样很容易生长。总之，首选MEM
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Assays of Glucose Entry, Glucose Transporter Amount, and Translocation
Glucose enters the cell by a carrier-mediated, facilitated diffusion mechanism,
2024-12-24 20 -
Large Scale Production of Nuclear Receptor Ligand-Binding Domains
The nuclear receptor superfamily is composed of over 150 inducible transcription
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Differentiation and Lineage Commitment of Murine Embryonic Stem Cells into Insulin Producing Cells
Pluripotent embryonic stem (ES) cells and induced pluripotent stem (iPS) cells r
2024-12-24 18
