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  • Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells

    Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells

    Embryonic stem cells (ESCs) are pluripotent cells derived from preimplantation e

    2025-01-07 41
  • Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion

    Ouabain Modulates Cell Contacts as well as Functions that Depend on Cell Adhesion

    Ouabain, a toxic of vegetal origin used for centuries to treat heart failure, ha

    2025-01-07 66
  • Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila

    Live Cell Approaches for Studying Kinetochore-Microtubule Interactions in Drosophila

    Kinetochores are essential for the proper positioning, movement and segregation

    2025-01-07 50
  • Analysis of Common Mitochondrial DNA Mutations by Allele-Specific Oligonucleotide and Southern Blot

    Analysis of Common Mitochondrial DNA Mutations by Allele-Specific Oligonucleotide and Southern Blot

    Mitochondrial disorders are clinically and genetically heterogeneous. There are

    2025-01-07 49
  • Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells

    Nuclear Transfer to Study the Nuclear Reprogramming of Human Stem Cells

    Research of stem cells will enable us to understand the development and function

    2025-01-07 53
  • 应力纤维(stree fibers)

    应力纤维(stree fibers)

    应力纤维又叫张力纤维,是真核细胞中广泛存在的一种较为稳定的束状纤维结构, 与骨骼肌中肌原纤维非常相像。应力纤维由大量平行排列的肌动蛋白组成,此外还含有其他在肌细

    2025-01-07 59
  • Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line

    Culture of Primary Adherent Cells and a Continuously Growing Nonadherent Cell Line

    Cell culture is an invaluable tool for investigators in numerous fields. It faci

    2025-01-07 44
  • Conventional Micropipette-Based Patch Clamp Techniques

    Conventional Micropipette-Based Patch Clamp Techniques

    The patch clamp technique revolutionized the study of ion channels and is consid

    2025-01-07 42
  • Scale-Up of Suspension and Anchorage-Dependent Animal Cells

    Scale-Up of Suspension and Anchorage-Dependent Animal Cells

    In this chapter, scale-up is described in a laboratory context (10–20 L), but th

    2025-01-07 37
  • On-Line Concentration of Environmental Pollutant Samples by Using Capillary Electrophoresis

    On-Line Concentration of Environmental Pollutant Samples by Using Capillary Electrophoresis

    This chapter reviews the theory and methodological developments of on-line conce

    2025-01-07 49
  • Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens

    Embryonic Stem Cells as a Source of Differentiated Neural Cells for Pharmacological Screens

    The process of bringing a new pharmacologically active drug to market is laborio

    2025-01-07 47
  • Measurement of Geminin Activity in Xenopus Egg Extracts

    Measurement of Geminin Activity in Xenopus Egg Extracts

    Geminin is an unstable protein that inhibits DNA replication by interfering with

    2025-01-07 47
  • Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics: Constructing GFP-Tagged Motor Enz

    Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics: Constructing GFP-Tagged Motor Enz

    The green fluorescent protein (GFP) from the Pacific Northwest jellyfish Aequore

    2025-01-07 50
  • 实验室最常用的移液枪你用对了吗?

    实验室最常用的移液枪你用对了吗?

    移液枪对我们医药生物研究人员来说是最熟悉不过了。可是,你真正了解它吗?你的使用方式是正确的吗?相信不少人听到这个问题后,已经开始回想自己平时的操作,也有一部分人

    2025-01-07 111
  • western-blot实验中ECL发光检测操作步骤

    western-blot实验中ECL发光检测操作步骤

    ECL Western特异发光检测试剂盒用于辣根过氧化物酶(HRP)标记的蛋白或核酸检测。下面以engreen的超敏型ECL发光液Enlight-Plus为例,

    2025-01-07 88