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Isolation of Non-muscle Actin
General Preparation1. Prepare buffers and have them cold. Make 50X stock of Buff
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Western Blot/Tissue Preparation
OverviewThis is a protocol for preparing whole cell lysate from tissue, for west
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一步法甲醛胶连检测蛋白质相互作用Protein Interactions Captured by Chemical Cross-linking: One-Step Cross-linking with
INTRODUCTIONThis protocol describes a method for chemical cross-linking of prote
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Protein extraction from whole tissues for IEF
Modified from that of Jay Thelen - University of Missouri-ColumbiaPhenol extract
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Western Blotting with Monoclonal Antibodies
Sample PreparationFor Protein Concentration Determination of Cell Culture Decant
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GST Fusion Protein Prep
Italics indicate optional steps especially useful for the analysis of untested p
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GSP融合蛋白的准备 GST Fusion Protein Prep
GST Fusion Protein PrepItalics indicate optional steps especially useful for the
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蛋白质和多肽MALDI-MS分析方法 General Method for MALDI-MS Analysis of Proteins and Peptides
ABSTRACTFor MALDI-TOF-MS analysis of proteins and peptides, samples are cocrysta
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Protein Expression and Purification Protocol
Step 1: Transform appropriate DNA plasmid into BL21(DE3) E. coli cells. These ce
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蛋白复合体直接酶消化法Direct Enzymatic Digestion of Protein Complexes
Direct Enzymatic Digestion of Protein ComplexesSherry Niessen, Ian McLeod and Jo
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Home-made Taq Polymerase Purification
Home-made Taq Polymerase PurificationI also have the bacteria containing the clo
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ELISA FOR DETECTING SECRETED ICAM-1
PurposeMaterialsNUNC-Immuno Plate IIF.Horseradish Peroxidase-Streptavidin (Zymed
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WESTERN BLOTTING USING CHEMILUMINESCENCE
REAGENTS ECL Western blotting kit (Amersham Life Science; cat# RPN2108): contai
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Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions In
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Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
IntroductionThis is an adaptation of the published procedure for RNAse treatment
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