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Bacterial Expression of Motors
MaterialsExpression plasmid (e.g.,pET/Ncd in BL21(DE3)pLysS host cells)ZB = 10 g
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单克隆抗体特异性识别的野生型谷胱甘肽-S-转移酶进展
Glutathione S-transferase omega 1 (GSTO-1) exists as both a wild-type (wt) and a
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GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
Margret B. Einarson and Elena N. Pugacheva Foxx Chase Cancer Center, Philadelphi
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Invitro transcription with yeast nuclear extract
In vitro transcription with yeast nuclear extractSteve HahnWear gloves throughou
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Protein Expression and Purification of RPA70-AB (181-422)
Comments: pSV281 RPA70AB Constructed by L.Mizoue; May '02.This plasmid encod
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大范围微管蛋白的制备
Large Scale Tubulin Preparation Tubulin is purified from bovine/porcine brain by
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常用核酸和氨基酸代码
Amino Acids TableHere is a list of the standard one-letter amino acid codes and
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蛋白质凝胶电泳凝胶的制备
【材料与试剂】(1)30%的丙烯酰胺:分别称取29g 丙烯酰胺,1g N,N -亚甲双丙烯酰胺加温热的去离子水60ml,加热至37℃溶解,补加水至终体积为100
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Native-PAGE常见问题分析
1. 非变性聚丙烯酰胺凝胶电泳时预电泳是怎么回事的?预电泳时要加6DNA上样缓冲液吗?电泳1-2小时再加结合反应产物吗?预电泳是除去凝胶中没有聚合的单体和双体和
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Native-PAGE实验方法
非变性聚丙烯酰胺凝胶和变性sds-page电泳在操作上基本上是相同的,只是非变性聚丙烯酰胺凝胶的配制和电泳缓冲液中不能含有变性剂如SDS等。一般蛋白进行非变性凝
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Native-PAGE和SDS-PAGE的比较
非变性凝胶电泳,也称为天然凝胶电泳,与非变性凝胶电泳最大的区别就在于蛋白在电泳过程中和电泳后都不会变性。最主要的有以下几点:1. 凝胶的配置中非变性凝胶不能加入
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Native-PAGE的基本分类
有三种常用的非变性聚丙烯酰胺凝胶电泳方法:blue native(BN-PAGE),clear native(CN-PAGE),quantitative pre
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非变性聚丙烯酰胺凝胶电泳(Native-PAGE)的分类
有三种常用的非变性聚丙烯酰胺凝胶电泳方法:blue native(BN-PAGE),clear native(CN-PAGE),quantitative pre
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非变性胶蛋白电泳
1. IntroductionSDS-PAGE (Section 2.2) is probably the most commonly used gel ele
2024-12-04 18 -
血清蛋白琼脂糖凝胶电泳
【原理】琼脂糖(agarose)是经过挑选,以质地较纯的琼脂(agar)作为原料而制成的。琼脂在化学上是由琼脂糖和琼脂胶组成的复合物。琼脂胶是一含有硫酸根和羟基
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