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Largescale nuclear extract preparation
Hattoti's protocol adapted to cell culture :Hattori M,Tugores A,Veloz L,Kari
2024-10-21 18 -
UV Quantitation of DNA
DNA absorbs ultraviolet light due to its highly conjugated nature. DNA may thus
2024-10-21 21 -
DNAPrecipitation
Phenol (removes protein)1.add equal volume of Phenol (= tris-saturated Phenol-Ch
2024-10-21 9 -
Protocols for ET recombination
1、Oligo design（1）The 5' end (the homology arm) - choose 42 or more (we usual
2024-10-21 9 -
Isopropanol precipitation-best time and temperature-M
Hi, does anyone know what is the best temperature and time for Isoprop precipita
2024-10-21 7 -
DNA ISOLATION FROM PRIMARY TUMORS VIA CRYOSECTIONS
- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sec
2024-10-21 9 -
Preparation of BAC DNA
The BAC clone from glycerol culture sublibrary is innoculated into 3 ml of LB wi
2024-10-21 9 -
SimplifiedDNAExtractionfromCellorTissue
PurposeDNA extraction without phenol extraction and centrifugation.Procedure1. L
2024-10-21 13 -
Tg 008 Southern Blotting
Transgenic Mouse and Gene Targeting FacilityTg 008 Southern BlottingMaterials an
2024-10-21 16 -
DNA Purification from Agarose Gels
1. Separate DNA fragments in an agarose gel cast with 0.5 mg/mL Ethidium bromide
2024-10-21 10 -
Oligo nucleotide Purification
SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g U
2024-10-21 9 -
Extraction from Cheek Cells DNA
Sample PreparationCheek cells are obtained by rinsing the mouth with 25mls of an
2024-10-21 19 -
Preparation of TonsilLysate
PurposeThe protocol describes how to prepare human tonsil lysate for use in puri
2024-10-21 10 -
Oligo nucleotide Purification
SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g U
2024-10-21 13 -
Extraction from Cheek Cells DNA
Sample PreparationCheek cells are obtained by rinsing the mouth with 25mls of an
2024-10-21 10
