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LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,1 mM PIPES,pH 6.8 at 4℃,250 ml for
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CARBONATE WESTERN TRANSFER SOLUTIONS
Dunn,Anal.Biochem.1986: 1571.5L1.0L10mM NaHCO31.26gchill to 4oC0.84g3mM Na2CO30.
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In vitro translation
Use Promega's nuclease-treated -Met (L4210)or -Cys (L4240).The lysate is the
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Northern Blotting：有效的RNA染色和转移
Northern Blotting技术专题(original protocol by R. M. Fourney, S. Miyakoshi, R. S. Da
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CAT Assay
Ref. Seed and Sheen, Gene 67, 271-277 (1988)Normal reaction is Chloramphenicol +
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Protein Interaction Analysis Using an IASYS Biosensor
IASYS-binding cuvette and getting KDImmobilization of ligands on cuvette surface
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小RNA的Northern BLOT
Northern Blotting技术专题Prepare glass plates for gel: Rinse plates with cold water
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Northern Blotting步骤和试剂配制(Breeden Lab)
Northern Blotting技术专题I. Electrophoresisclean gel box with NaOH and/or SDS, 2 hou
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Too many bands on a Western blot
Western- or immunoblotting is a commonly employed technique for the detection of
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Labeling Vinculin with TRITC
Materials1.200 mM K-borate,pH 9.0,10 ml.2.100 mM lysine,100 mM K-borate,pH 9.0,1
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Western Blots
1)Run protein gel.Set up blot:2)Prepare two tupperware containers,one with dH2O
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美国实验室WESTERN BLOT 方法
WESTERN BLOT PROTOCOL In a Western blot, proteins that are separated on polyacry
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Western Blotting - Antibodies
1. Load gel in apparatus as described by manufacturer.2. Transfer proteins accor
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Gel Mobility Shift Analysis
Material/reagents:• 2X binding buffer: 20 mM Tris pH 7.5 100 mM NaCl
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Labeling Muscle Actin with N-(1-pyrene)iodoacetamide
Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml
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