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Labeling oligonucleotides with 32P ATP
Wear gloves throughout and work in radiation area. Monitor area before and after
2024-10-09 19 -
Genomic DNA Labeling Protocol
We typically use 0.5ug of E. coli genomic DNA in a labeling reaction for each hy
2024-10-09 15 -
Removal of 32P-ATP from Oligonucleoi
1. Prepare Whatman DE-52 according to manufacturers specifications. Equilibrate
2024-10-09 15 -
Labeling oligonucleotides with 32P ATP
Wear gloves throughout and work in radiation area.Monitor area before and after
2024-10-09 16 -
Fill-in Labeling of DNA Fragments
This protocol was designed to generate directionally end-labeled probes for DNas
2024-10-09 14 -
Screen ES cells by Southern Blot
Digest DNA in 96-well plateTo each well add:4μl 10Xbuffer4μl Enzyme0.4μl Spermid
2024-10-09 15 -
Tail Chop Southern Protocol
About 1/2 to 1 cm of tail should be cut from properly marked mice (using toe or
2024-10-09 13 -
SSC-Molecular Biology
Hi,Can you tell me what do "SSC" in a southern blot, how he act on DNA
2024-10-09 12 -
Southern Blotting(Fred Hutchinson Cancer Research Center)
DNA Transfer1. Depurination of DNA fragments: Wash gel in 0.25 M HCl 5' (sma
2024-10-09 13 -
Benton Davis Blots
Day 1Prepare in advance:Chill plates containing bacteriophage-lysed E. coli for
2024-10-09 16 -
SOUTHERN BLOTTING OF GENOMIC DNA
1.Digest DNA:• 1.5 μg DNA in a 25 μI reaction containing BSA• 3 to 5 units for 5
2024-10-09 20 -
Southern Blotting-Molecular Biology
Has anyone any experience of using a semi dry blotter for Southern blotting. At
2024-10-09 14 -
RAPID BIDIRECTIONAL SOUTHERN BLOT FOR CLONED DNA
A. Process gel1. Electrophorese sample in agarose gel, 0.5 to 1 cm thick, 0.5-1.
2024-10-09 17 -
Alkaline Southern Blotting Procedure
Author: Suzanne GerttulaDate: March 14,19941.Digest between 1 and 10 μg Genomic
2024-10-09 18 -
Non-radioactive Probes
Non-radioactive ProbesI. Via random hexamers1. Solutions:10X hexa nt mix*:500 mM
2024-10-09 13
