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Tg 008 Southern Blotting
Transgenic Mouse and Gene Targeting FacilityTg 008 Southern BlottingMaterials an
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Southern Hybridization Experiment Kit
This kit contains materials for six groups to perform Southern transfer and hybr
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SOUTHERN BLOTTING
Materials:Whatman 3 mm Blotting Papernitrocellulose (Schleicher & Schuell, Amers
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Southern Analysis
Southern AnalysisProcedure (for analysis of genomic DNA or ordering of clones):1
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非放射性Dig－dUTP
[原理]DNA是通过异羟基洋地黄毒苷（digoxigenin，Dig）配基标记的脱氧尿嘧啶核苷三磷酸（dUTP）随机插入结合而被标记。dUTP通过间臂连结类固
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Genomic Southern Blot
SolutionsProtocol:Digest 5-10 μg genomic DNA overnight with restriction enzyme o
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Southern Blot Protocol
Day 11. Digest DNA for 6 hours (or overnight)BSA 10 mg/ml 0.5 22.65 μl of 10 μg
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Southern Blot Analysis(from Baker lab, university of Florida)
DNA PrepPrepare DNA via your favorite method.You may find a protocol underMini Y
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Southern印迹技术
Southern印迹技术关键词： 印迹技术来源： 互联网实验原理:Southern印迹是将DNA片断从电泳凝胶上直接转移至膜支持物（如硝酸纤维素膜、尼龙膜）上，
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Random Primer DNA labeling
DESCRIPTIONLabeling of DNA by random oligonucleotide-primed synthesis is based o
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Southern Blotting: DNA Transfer
1. Depurination of DNA fragments: Wash gel in 0.25 M HCl 5' (small gels), 7&
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Southern 转印分析
在胶体中的DNA 可利用毛细现象的作用将DNA 牵引转印至覆盖在胶片上的滤膜，经烘烤或UV 照射后，可使DNA 固定在膜上，以进行探针的杂合（hybridiza
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小麦Southern杂交分析方法
预杂交液的制备：根据要杂交的膜的数量配制预杂交液，每25 ml预杂交液（1~2张膜）的成分组成如下：H2O15 ml20SSPE6.25 ml50Denhard
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Southern杂交鉴定方法
1. 取10ul待测DNA，于一定浓度的琼脂糖凝胶上进行电泳。(20mL，1.0%凝胶)2. 凝胶用溴化乙锭染色，切掉凝胶四周多余部分，并在凝胶的一角作一记号，
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双链DNA探针切口平移法
当双链DNA 分子的一条链上产生切口时,E.coli DNA 聚合酶Ⅰ就可将核苷酸连接到切口的3'羟基末端。同时该酶具有从5'3'的核酸
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