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Cohesive End Ligation
1) The ligation mixture contains the following:vector (~100 ng) insert (equimo
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VHCDR3基因文库和VL基因的扩增
[器材和试剂]● PCR试剂和设备● Geneclean试剂盒（Qbiogene）● VHFOR随机引物（见下文），10pmol/ul● LMB3引物●
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DNA“分子笼”成纳米级药物“递送车”
据美国物理学家组织网7月4日报道，最近，牛津大学科学家首次开发出一种由DNA(脱氧核糖核酸)制造的分子“笼子”，能进入活细胞内部并在其中生存，由此可能带来一种有
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cDNA和染色体组文库的筛选
Choice of bacterial strain:The bacterial E. coli strains we use when plating out
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Cosmid Library
METHOD:1.Isolate chromosomal DNA by using the CTAB protocols in current Pr M R.2
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Growing up of BAC clones
1. BACs are usually supplied as stabs in agar (2.5 ml screw-top tubes filled wit
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再扩增VH基因文库添加3‘限制性位点并克隆创建VH基因节段文库
[器材和试剂]● PCR试剂和设备● Wlzard PCR纯化试剂盒（Promega）● PVHlOR2引物： 5'-GAG TCA TTC T
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Screening lambda cDNA or genomic libraries
MediaNeed LB plates (9 cm or 14 cm)to plate the library and do secondary screens
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Yeast Two-Hybrid Screen with Library and Bait
YEAST TWO-HYBRID SCREEN WITH LIBRARY AND BAIT(The following protocol is for use
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freeshotgun sequencing library from miniprep BAC DNA
Miniprep of BAC DNA1.Inoculate 4 - 8 tubes of 3ml/tube LB with 15μg/ml chloramph
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从噬菌体文库中克隆基因
The overall sequence of events is:• Titer and plate out phage • Lift plaques ont
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美编制出光合作用597个编码基因蛋白目录
据美国物理学家组织网近日报道，美国卡内基学院、加利福尼亚大学洛杉矶分校与美国能源部联合研究院利用先进的计算机工具，分析了28种植物中与光合作用相关的基因组，编制
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Shotgun Library
This protocol is intended to make shotgun libraries from BACS that are to be ful
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Linker Ligation
Linker Ligation (with T4 ) DNA LigaseIn a microcentrifuge tube prepare a solutio
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PCR剪接VHCDR3基因文库和VL基因构建scFv基因文库
[器材和试剂]Winard PCR纯化试剂盒 （Promega）PCR试剂和设备用于连接scFv和pHENl DNA以及将scFv文库电转化到大肠杆菌TGl株的
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