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  • DNA电泳带型分析

    DNA电泳带型分析

    DNA电泳需要进行带型分析,在实验过程中常会发现所得的带型出现在这样那样的问题。在此,对DNA带型做了相应的分析。带型原因分析解决办法弱带或无带上样的DNA量不

    2024-09-26 26
  • 琼脂糖凝胶电泳迁移速率的影响因素

    琼脂糖凝胶电泳迁移速率的影响因素

    1、DNA的分子大小及构型不同构型DNA的移动速度次序为:供价闭环DNA(covalently closed circular,cccDNA)>直线DNA

    2024-09-26 32
  • 凝胶迁移或电泳迁移率实验(EMSA)

    凝胶迁移或电泳迁移率实验(EMSA)

    凝胶迁移或电泳迁移率实验(EMSA)是一种研究DNA结合蛋白和其相关的DNA结合序列相互作用的技术,可用于定性和定量分析。这一技术最初用于研究DNA结合蛋白,目

    2024-09-26 41
  • Sending Sera to Testing for Mouse Pathogens.

    Sending Sera to Testing for Mouse Pathogens.

    Sending Sera to Testing for Mouse Pathogens.Bleed mice: Obtain 300-400µl from mi

    2024-09-25 24
  • Karyotyping Xenopus embryos

    Karyotyping Xenopus embryos

    This protocol was provided to us from the Grainger lab and modified by M. Khokha

    2024-09-25 24
  • ES injection pipette manufacture

    ES injection pipette manufacture

    ES injection pipette manufactureThe following is an outline of the method that I

    2024-09-25 27
  • DIFFERENTIAL DISPLAY

    DIFFERENTIAL DISPLAY

    Comparible RNAs were isolated from different treatments using my favorite method

    2024-09-25 21
  • Differential Display Technical notes

    Differential Display Technical notes

    Optimal arbitrary primer length for Differential Display The concept of differen

    2024-09-25 20
  • Superovulation of Mice

    Superovulation of Mice

    Given each mouse 0.05 ml PMSG (Straight from vial), administered i/p at 12 o'

    2024-09-25 26
  • Transgenic Animals

    Transgenic Animals

    Transgenic AnimalsThe term “transgenic animals” describes animals whose chromoso

    2024-09-25 21
  • DNA Preparation for Microinjection or Electroporation

    DNA Preparation for Microinjection or Electroporation

    This protocol was developed by Jan Parker-Thornburg at The Ohio State Universtiy

    2024-09-25 31
  • Molecular Analysis and Results - DNA

    Molecular Analysis and Results - DNA

    Theory of CGH Comparative genomic hybridization (CGH) is a fairly new molecular

    2024-09-25 22
  • Microinjection of dsRNA into Mouse Oocytes and Early Embryos

    Microinjection of dsRNA into Mouse Oocytes and Early Embryos

    Microinjection of dsRNA into Mouse Oocytes and Early EmbryosPaula Stein and Petr

    2024-09-25 24
  • PROTOCOLES MICROSATELLITES / MICROSATELLITE PROTOCOLS

    PROTOCOLES MICROSATELLITES / MICROSATELLITE PROTOCOLS

    Microsatellite markers: isolation with non-radioactive probes and amplification

    2024-09-25 22
  • Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)

    Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)

    PCR Protocol10X PCR Buffer1.0MgCl2 (2.0mM)0.8µldNTPS mix*0.8µlPrimer-F (µg/µl)0.

    2024-09-25 27