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Plasmid Quickpreps
1.grow up single colony in 1.5 ml LB/antibiotic ovn @37℃ .2.pour into tube, spin
2024-10-19 14 -
Preparation of linear polyacrylamide
Preparation of linear polyacrylamide used as carrier in ethanol precipitation of
2024-10-19 12 -
OLIGO PURIFICATION on acrylamide gels
GEL PURIFICATION OF OLIGONUCLEOTIDES(Adapted from the method of Mark Fortini)1.
2024-10-19 19 -
Phlebotomus Sandflies的DNA的提取
1.Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl ,10 mM EDTA,50
2024-10-19 17 -
Plant Total DNA Isolation
This protocol describes a method for isolating DNA from plant tissue.Procedure1.
2024-10-19 15 -
Improved Alcohol Precipitation of DNA
ECKDescriptionThis method was suggested in a BRL "Focus" article sever
2024-10-19 17 -
DNA and RNA EXTRACTIONS
A protocol / method / schedule /procedure for extraction / isolation of both DNA
2024-10-19 18 -
Large scale nuclear extract preparation
Hattoti's protocol adapted to cell culture :Hattori M,Tugores A,Veloz L,Kari
2024-10-19 18 -
how to clean DNA10KB- Molecular Biology
I tried to clean a restriction product >10kb before ligation by a gel extract
2024-10-19 19 -
Removal of 32P-ATP from Oligonucleoi
1. Prepare Whatman DE-52 according to manufacturers specifications. Equilibrate
2024-10-19 21 -
DNA重组实验中常用的技术(一）
（一）质粒DNA 的提取及鉴定1．收获细菌（1）将2ml含相应抗生素的LB液体培养基加入到通气良好的15ml的试管中，接入一单菌落，于37℃剧烈振荡培养过夜。（
2024-10-19 17 -
Phenol-based Method for the Isolatio
Phenol-based Method for the Isolation of DNA Fragments from Low-Melting Temperat
2024-10-19 27 -
Triton-Prep Method for bacterial DNA Purification
1.Grow 5 ( large scale-15ml culture). Harvest in single eppendorf tube (or 15 ml
2024-10-19 23 -
PlasmidMini-prepProtocol
1. Spin down 1.5 ml of overnight culture in 2ml or 1.5ml tube for 1 minute on hi
2024-10-19 19 -
Phenol extraction of DNA samples
(adapted from Bruce A.Roe,Department of Chemistry and Biochemistry,The Universit
2024-10-19 20
