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Benton Davis Blots
Day 1Prepare in advance:Chill plates containing bacteriophage-lysed E. coli for
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SOUTHERN BLOTTING OF GENOMIC DNA
1.Digest DNA:• 1.5 μg DNA in a 25 μI reaction containing BSA• 3 to 5 units for 5
2024-10-09 30 -
Southern Blotting-Molecular Biology
Has anyone any experience of using a semi dry blotter for Southern blotting. At
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RAPID BIDIRECTIONAL SOUTHERN BLOT FOR CLONED DNA
A. Process gel1. Electrophorese sample in agarose gel, 0.5 to 1 cm thick, 0.5-1.
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Alkaline Southern Blotting Procedure
Author: Suzanne GerttulaDate: March 14,19941.Digest between 1 and 10 μg Genomic
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Non-radioactive Probes
Non-radioactive ProbesI. Via random hexamers1. Solutions:10X hexa nt mix*:500 mM
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TMCF Protocols: Southern Blotting
BUFFERS:1) Prehybridization/hybridization buffer1% SDS6X SSC10 % dextran sulfate
2024-10-09 26 -
southern blots-Molecular Biology
Hi can anyone answer thisI am having problems with my southern hybridization.I a
2024-10-09 30 -
RNA/Zeta Probe Dot Blotting protocol
RNA/Zeta Probe Dot Blotting protocol1)Make up RNA (up to 20μg)dissolved in steri
2024-10-09 29 -
Southern Blot
1. Run gel (0.8 -1.0% agarose is best). For yeast chromosomal Southerns, digest
2024-10-09 31 -
genomic DNA digest-Molecular Biology
I am trying to digest mouse genomic DNA for a southern blot. When digested with
2024-10-09 27 -
Radiolabeling of probes for electrophoretic mobility shift assays
Materials:g -32 P ATP (New England Nuclear/Perkin Elmer, CAT# NEG-035C)“Upper s
2024-10-09 26 -
Southern protocol-Molecular Biology
Hi everyone,i'm looking for a good southern blot protocol with all the secre
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Analysis of Genomic DNA by Southern Hybridization
Analysis of Genomic DNA by Southern HybridizationSelect several independent BAC
2024-10-09 28 -
Southern Blot using Neutral Transfer
-Elaine Pinheiro and Aurora Burds ConnorMIT, Jan2006This protocol works very wel
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