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Purification of Antibodies Using Protein A-Sepharose and FPLC
Protein A chromatography is a type of affinity chromatography that relies on the
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Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography
Immobilized metal affinity chromatography (IMAC), introduced in 1975 (1 ), relie
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Purification of Antimicrobial Peptides from Human Skin
Human skin is a rich source of human antimicrobial peptides. Its cellular source
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Purification of C1q Receptors and Functional Analysis
The recognition subunit of C1, C1q, has emerged as an important player in variou
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Purification of Capsular Polysaccharide
Meningococcus is an aerobic, fastidious, Gram-negative diplococcus that is found
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Purification of Chemokines from Natural Sources
Chemokines (e.g., IL-8) were originally identified as chemotactic proteins obtai
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Purification of Complement Components, Regulators, and Receptors by Classical Methods
The aim of this chapter is to describe methods for purification of the individua
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Purification of DNA Topoisomerases and Inhibition by Fluoroquinolones
Fluoroquinolones act by inhibiting the essential type II topoisomerases, DNA gyr
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Purification of Glycoproteins and Their Use as Immunogens
Glycosylation is a major posttranslational modification that produces heterogene
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Purification of Human Complement Protein C5
Complement C5 is cleaved by proteolysis in the terminal phase of complement acti
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Purification of Human Monoclonal Antibodies and Their Fragments
This chapter summarizes the most common chromatographic mAb and mAb fragment pur
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Purification of Human NK Cell Developmental Intermediates from Lymph Nodes and Tonsils
Accumulating data indicate that human natural killer (NK) cells undergo terminal
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Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
Most T lymphocytes of the immune system differentiate within the thymus along th
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Purification of Immunoglobulin G (IgG)
Several immunological procedures can be successfully carried out using nonpurifi
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Purification of Large Cytosolic Proteases for In Vitro Assays: 20S and 26S Proteasomes
Proteasomes are the main cytosolic proteases responsible for generating peptides
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