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Superovulation of Mice
Given each mouse 0.05 ml PMSG (Straight from vial), administered i/p at 12 o'
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Transgenic Animals
Transgenic AnimalsThe term “transgenic animals” describes animals whose chromoso
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DNA Preparation for Microinjection or Electroporation
This protocol was developed by Jan Parker-Thornburg at The Ohio State Universtiy
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Molecular Analysis and Results - DNA
Theory of CGH Comparative genomic hybridization (CGH) is a fairly new molecular
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Microinjection of dsRNA into Mouse Oocytes and Early Embryos
Microinjection of dsRNA into Mouse Oocytes and Early EmbryosPaula Stein and Petr
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PROTOCOLES MICROSATELLITES / MICROSATELLITE PROTOCOLS
Microsatellite markers: isolation with non-radioactive probes and amplification
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Mutation Detection By Single-Strand Conformational Polymorphism (SSCP)
PCR Protocol10X PCR Buffer1.0MgCl2 (2.0mM)0.8µldNTPS mix*0.8µlPrimer-F (µg/µl)0.
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PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS
Cut 10-20X 10 um sections of formalin fixed paraffin samples into eppendorf tube
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Isolation of complexes from serum
Complexes can be isolated from serum by centrifugation on either a sucrose or Pe
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Targeted Differential Display
This method was successful in our lab using prostate tissue and for our specific
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Transgenic Core Facility
The purity of the transgene DNA used for microinjection is critical for the succ
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DIFFERENTIAL DISPLAY
DIFFERENTIAL DISPLAY Comparible RNAs were isolated from different treatments usi
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DNA preparation from mouse tails
General precautions to be followed during handling of any genomic DNA sample:Avo
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Mating transgenic mice
Determine the age of your mice. Mice will usually not breed if they are younger
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Transgenic Outline: Mice and Rats
Thom Saunders with any questions or Meet the Staff.review of reporter molecules
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