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Practical Tips for Handling RNA
Phenol ExtractionOne of the most common molecular biology lab procedures, phenol
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Phenol Extraction of rRNA (Rat liver)
Phenol Extraction of rRNA (Rat liver)LEVEL II *** READ THROUGH ALL CAUTIONS BEFO
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Water Treatment with DEPC
Add 0.1% diethylpyrocarbonate (DEPC) to water, mix overnight, and then autoclave
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Northern Blotting
Northern BlottingAdapted from Molecular Cloning (Maniatis), Current Protocols in
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Top Ten Ways to Ensure Valid RNAi Data
１.Confirm results with a second or third siRNA to the same target. One of the
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Northern protocol(RULES FOR RNA WORK)
1. Wear gloves at all time including filling pipet tip in racks, filling
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How to prepare Molecular Biology grade glycogen
How to prepare Molecular Biology grade glycogenAuthor: Roberto SalviSource: Cont
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Purification of poly(A)+ RNA from total RNA
Purification of poly(A)+ RNA from total RNA0. (Optional)Before using Oligotex-dT
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RNA interference：The short answer
One way of seeing what a gene does is to block its messenger RNA and note the ef
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RNAi——制备siRNAs的几种方法
越来越多的研究人员开始采用小分子干扰RNA（small interfering RNAs，siRNAs)来抑制特定的哺乳动物基因表达。siRNA是一种短片断双链
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Preparation of single-stranded probes from cloned cDNA
a) 2-5 µg of plasmid DNA containing the cDNA insert is linearized using an appro
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IN SITU HYBRIDIZATION ON FROZEN SECTIONS
Remove slides from freezer, thaw for 5 min. at 55C. Fix 10 min. in 4% paraformal
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如何确定RNA质量的经验谈！
1） 检测RNA溶液的吸光度 280、320、230、260nm下的吸光度分别代表了核酸、背景（溶液浑浊度）、盐浓度和蛋白等有机物的值。一般的，我们只看OD26
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How to Maintain an RNase-free Lab
How to Maintain an RNase-free LabLet's admit it, everyone who has worked wit
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35S-RIBOPROBE SYNTHESIS FOR ISOTOPIC In Situ HYBRIDIZATION
Pipet 12.5µl 35S-UTP (1200 Ci/mmol) into 1.5ml microfuge tube. Final concentrati
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