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  • Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads

    Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads

    Although phospho-specific primary antibodies used in immunohistochemistry (IHC)

    2025-04-09 5
  • Expression of Eukaryotic Cytochromes P450 in E. coli

    Expression of Eukaryotic Cytochromes P450 in E. coli

    Although much has been learned from the study of relatively abundant cytochromes

    2025-04-09 13
  • Micellar Polymer Encapsulation of Enzymes

    Micellar Polymer Encapsulation of Enzymes

    Although enzymes are highly efficient and selective catalysts, there have been p

    2025-04-09 3
  • Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations

    Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations

    Although changes in both pHin and [Ca2+ ]i have been observed in response to a v

    2025-04-09 4
  • In Vitro Assays to Analyze Translocation of the Model Secretory Preprotein Alkaline Phosphatase

    In Vitro Assays to Analyze Translocation of the Model Secretory Preprotein Alkaline Phosphatase

    Almost one-third of the proteins synthesized in the cytosol of cells ends up in

    2025-04-09 7
  • Selecting Allosteric Ribozymes

    Selecting Allosteric Ribozymes

    Allosteric ribozymes can be designed to respond to virtually any molecule of cho

    2025-04-09 11
  • (E)-Alkene Peptide Bond Isosteres by Cuprate Opening of Vinyl Aziridines

    (E)-Alkene Peptide Bond Isosteres by Cuprate Opening of Vinyl Aziridines

    Alkene isosteres are important nonhydrolyzable and rigidified analogs of peptide

    2025-04-09 14
  • Experimental assessment of the role of acetaldehyde in alcoholic cardiomyopathy

    Experimental assessment of the role of acetaldehyde in alcoholic cardiomyopathy

    Alcoholism is one of the major causes of non-ischemic heart damage. The myopathi

    2025-04-09 6
  • Intragastric Ethanol Infusion Model in Rodents

    Intragastric Ethanol Infusion Model in Rodents

    Alcohol-associated life-style disease, as exemplified by alcoholic liver disease

    2025-04-09 8
  • Assessment of Intracellular Mucin Content In Vivo

    Assessment of Intracellular Mucin Content In Vivo

    Airway mucus presents a first line of defense against inhaled materials. It also

    2025-04-09 5
  • Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-No

    Immunochemical Detection of a Fluorophore Derived from the Lipid Peroxidation Product 4-Hydroxy-2-No

    Aging and the progression of certain degenerative diseases are accompanied by in

    2025-04-09 10
  • Horizontal SDS-PAGE for IPG-Dalt

    Horizontal SDS-PAGE for IPG-Dalt

    After IEF-IPG (see Chapters 21 –24 ), the IPG gel strips are equilibrated in the

    2025-04-09 9
  • Peptide Release, Side-Chain Deprotection, Work-Up, and Isolation

    Peptide Release, Side-Chain Deprotection, Work-Up, and Isolation

    After having successfully synthesized a peptide, it has to be released from the

    2025-04-09 8
  • High-Throughput Affinity Mass Spectrometry

    High-Throughput Affinity Mass Spectrometry

    Affinity mass spectrometry (AMS) is a proteomics approach for selectively isolat

    2025-04-09 4
  • Affinity Capillary Electrophoresis to Examine Receptor-Ligand Interactions

    Affinity Capillary Electrophoresis to Examine Receptor-Ligand Interactions

    Afffinity capillary electrophoresis (ACE) is a new analytical technique that has

    2025-04-09 4