-
The Use of TAGZyme for the Efficient Removal of N-Terminal His-Tags
The use of affinity tags and especially histidine tags (His-tags) has become wid
2025-03-07 16 -
Simplified Calcium Phosphate Coprecipitation
Materials 2x HEPES: 8 g NaCl, 0.105g NaHPO4 , 6.5 g HEPES, H2O to 500 mL, pH 6.9
2025-03-07 16 -
肽酰转移酶(peptidyl transferase)
肽酰转移酶是催化肽键形成的酶。在蛋白质合成过程中，它催化核糖体A位tRNA上末端氨基酸的氨基与P位肽酰-tRNA上氨基酸的羧基间形成肽键。其结果,使A位的氨酰-
2025-03-07 22 -
MudPIT Analysis: Application to Human Heart Tissue
Although two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) has been u
2025-03-07 16 -
Assays to Measure Stress-Activated MAPK Activity
Mitogen-activated protein kinases (MAPKs) are activated by a wide variety of cel
2025-03-07 19 -
Classical Human Epidermal Keratinocyte Cell Culture
It has been more than 30 years since the serial cultivation of human keratinocyt
2025-03-07 14 -
MTS检测细胞生长
1． 培养 IL-2 依赖细胞株 CTLL-2 细胞或 HT-2 细胞（检测 IL-2 ），或者 IL-3 依赖细胞株 FDC-P1 细胞或 FL5.12 细胞
2025-03-07 11 -
Measurements of White Adipose Tissue Metabolism by Microdialysis Technique
The microdialysis method was introduced 25 yr ago to measure neurotransmitter co
2025-03-07 14 -
Liquid Chromatography MALDI MS/MS for Membrane Proteome Analysis
Liquid chromatography (LC) can be combined with matrix-assisted laser-desorption
2025-03-07 11 -
In Vitro Matrigel Angiogenesis Assays
A variety of in vivo and in vitro methods have been used to study angiogenesis,
2025-03-07 15 -
Locked Nucleic Acid Aptamers
The aptamer technology has been introduced in the early 1990s. With this techniq
2025-03-07 17 -
核仁组织区（nucleolus organiting region，NOR）
参与形成核仁时的染色质区，核仁从核仁组织区部位产生，同时与该区紧密相连。具有核仁组织区的染色体称核仁染色体。核仁组织区定位在核仁染色体次缢痕部位。对人来说，在1
2025-03-07 18 -
原核融合(pronuclei fussion)
精细胞的细胞核进入卵细胞质后，包装紧密的染色质开始解旋。精子的细胞核膜解体形成小的囊泡，并立即与精子细胞核脱离，形式没有核被膜的精子染色质，但很快形成新的核被膜
2025-03-07 18 -
分裂间期
真核细胞在细胞周期中，由上次细胞分裂结束到下次细胞分裂开始的持续时间。间期细胞进行旺盛的生物合成和生长，是细胞进入有丝分裂期的重要准备阶段。间期可划分为： G1
2025-03-07 13 -
细胞遗传学――原位杂交(ISH)
In Situ Hybridization・ In Situ Hybridization (jsmith1@po-box.mcgill.ca )
2025-03-07 14
