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悬浮细胞转染后筛选问题求助
问：大家好，我第一次作转染，是悬浮细胞，已经在园子里看了好多这方面的资料，收获很大，可还是几个问题要问大家：1）我打算用24孔板作转染，之后的筛选过程中肯定会有
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逆转录病毒包装
问：大家好，请问有人做过逆转录病毒包装吗，我最近在用PT67包装病毒，按照说明书上的要求，在细胞长到70%左右汇合时加入质粒和脂质体和无血清培养液培养4小时后更
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G418筛选实验相关问题
问：G418筛选预实验用未转染的细胞做，选10-14天内细胞死亡的最小浓度，然后将更高一级别的浓度应用于转染的细胞，是这样吗？答：G418是一种细胞毒性药物（氨
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cos-7的转染
问：cos-7的转染1、细胞的培养：我们在培养细胞时发现该细胞长的很快，不到2天的时间就长满了；消化的时候老是成块的掉，如果消化时间加长，细胞也能成单个的，但是
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Gene Gun Delivery Systems for Cancer Vaccine Approaches
Gene-based immunization with transgenic DNA vectors expressing tumor-associated
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微胶囊造粒技术在细胞移植，药物和食品研究中的成功应用
微胶囊造粒技术成功用于细胞移植治疗疾病研究：不久前，BBC报道了一则伦敦医生采用微胶囊肝细胞移植手术成功救治了一位患肝脏疾病的重症男婴的事件。肝细胞移植前首先通
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细胞凋亡后beta－actin会降解吗？
beta actin is a type of cell skeleton protein.Its gene is a house keeping gene.I
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Clonogenic Cell Survival Assay
The clonogenic cell survival assay determines the ability of a cell to prolifera
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SDS -PAGE and Western Blotting Techniques
The goal of Western blotting, or more correctly, immunoblotting, is to identify
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Sulforhodamine B Assay and Chemosensitivity
The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measu
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The Concept of Multiple-Target Anti-miRNA Antisense Oligonucleotide Technology
The multiple-target AMO technology or MT-AMO technology is an innovative strateg
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Molecular Characterization of Human Papillomaviruses by PCR and In Situ Hybridization
The goal of early detection and screening is the diagnosis and treatment of canc
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Measuring MDR-1 by Quantitative RT-PCR
Accurate determination of the expression of P-glycoprotein and its encoding mRNA
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The Use of M-FISH and M-BAND to Define Chromosome Abnormalities
Multicolour fluorescence in situ hybridisation (M-FISH) and multicolour banding
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Antibody Targeting of Nanoparticles to Tumor-Specific Receptors: Immunoliposomes
Immunoliposomes generated by coupling of antibodies to the liposomal surface all
