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蛋白质检测-Western Blot
蛋白质检测- Western Blot 1. 把聚丙烯酰胺凝胶中的蛋白质电泳转移到硝酸纤维膜上。1) 转移缓冲液洗涤凝胶和硝酸纤维素膜,将硝酸纤维素膜铺在凝胶上
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Rule-Based Modeling of Signal Transduction: A Primer
Biological cells accomplish their physiological functions using interconnected n
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Epitope Mapping
The region of an antigen that interacts with an antibody is defined as an epitop
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Techniques to Measure Nucleic Acid-Protein Binding and Specificity: Nuclear Extract Preparations, DN
DNA-protein interactions are the basis for the molecular mechanisms responsible
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Immunoelectron Microscopy of Cryofixed Freeze-Substituted Mammalian Tissue Culture Cells
Mammalian tissue cultured cells are widely used in cell biology research. Immuno
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Transcriptome-Based Identification of Candidate Membrane Proteins
A full understanding of leukocyte responses to external stimuli requires knowled
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Sequence-Specific Primed PCR (PCR-SSP) Typing of HLA Class I and Class II Alleles
For donor selection in hematopoetic stem cell transplantation, two-digit sequenc
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Transcriptome-Based Identification of Candidate Membrane Proteins
A full understanding of leukocyte responses to external stimuli requires knowled
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Sequence-Specific Primed PCR (PCR-SSP) Typing of HLA Class I and Class II Alleles
For donor selection in hematopoetic stem cell transplantation, two-digit sequenc
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Treatment of Spontaneous Soft Tissue Sarcomas in Cat
Preclinical experiments performed on mice clearly showed that electrochemotherap
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侧群干细胞(sp细胞)的分离与分选
准备工作: DF12 加入0.1%BSA,100ml,取10ml冰浴,40ml放入孵箱;其余的放入4度; DNase I 100*; PI:50ug/ml; H
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转铁蛋白(transferrin)
转铁蛋白是血液中一种主要的糖蛋白, 负责将肝组织(是铁贮藏的主要场所)的铁向其它组织的细胞运输。没有结合铁的转铁蛋白称作脱铁转铁蛋白(apotransferri
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内分泌信号(endocrine signaling)
由内分泌细胞合成并分泌到细胞外进行信号传导的分子称为内分泌信号。一般为激素类物质。这类信号分子通讯方式的距离最远,覆盖整个生物体。内分泌信号的激素有三种类型:蛋
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转胞吞作用(transcytosis)
转胞吞作用是一种特殊的内吞作用,受体和配体在内吞中并未作任何处理,只是经细胞内转运到相反的方向, 然后通过胞吐作用, 将内吞物释放到细胞外,这种内吞主要发生在极
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显微镜放射自显影样品的制备及观察
相关专题 显微放射自显影就是运用放射性标记化合物掺入生物样品,以及用核乳胶记录生物样品内放射性同位素释放核射线的技术。根据放射性标记化合物的性质和银粒的分布部