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Monitoring Protein Kinase A Activities Using Expressed Substrate in Live Cells
Protein kinase A (PKA) activity is regulated by intracellular cyclic adenosine m
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Confirmation of the Presence of Drugs of Abuse in Urine
Urine screenings for drugs of abuse are performed for many reasons, two of the m
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小分子RNA(small RNA)
存在于真核生物细胞核和细胞质中,它们的长度为100到300个碱基(酵母中最长的约1000个碱基)。多的每个细胞中可含有105 ~106 个这种RNA分子,少的则
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Thin-Layer Gel Filtration: Determination of Protein Molecular Weights
For many years Sephadex gel filtration of proteins has been a valuable tool for
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Oocyte Extracts for the Study of Meiotic M-M Transition
In meiotic cell cycles, meiosis I (MI) is followed by meiosis II (Mil) without a
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胚胎干细胞培养操作规程
维持ES细胞处于未分化状态:ES细胞培养用含有ESGRO(白血病抑制因子)的高糖培养基来阻止细胞的分化。为细胞提供包被有0.1%明胶的平板作为粘附细胞的基质。建
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Rockland Dr.Moncada 分享: 如何获得更漂亮的流式结果
1. 如何降低背景 流式细胞实验优化过程中的一个关键步骤是试剂(抗体)的滴定。为了达到最佳效果,实验者必须确定抗原结合达到饱和所需抗体的最低量。这将帮助实验者提
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Imaging Hematopoietic Stem Cells in the Marrow of Long Bones In Vivo
Hematopoietic stem and progenitor cells (HSPCs) are located in the bone marrow i
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In Situ Hybridization of Whole-Mount Embryos
Since the early analyses of gene expression in the Drosophila embryo (1 ), whole
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Autoradiography of 1-and 2-Adrenoceptors
Receptor autoradiography is derived from receptor binding techniques that were o
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胚胎干细胞培养
Media and Solution required for ES Cell Culture (Bowtell Lab)Routine Culturing o
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肌动蛋白(actin)
肌动蛋白是微丝的结构蛋白, 以两种形式存在, 即单体和多聚体。单体的肌动蛋白是由一条多肽链构成的球形分子, 又称球状肌动蛋白(globular actin, G
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A Mammalian Cell-Based Assay for Screening Inhibitors of RNA Cleavage
RNA cleavage is a catalytic reaction which defines many types of RNA processing
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细胞培养中血清使用的常见问题
1.保存血清最好的办法? 我们建议血清应保存在-5℃至-20℃。若你一次无法用完一瓶,建议您无菌分装血清至恰当的灭菌容器内,再放回冷冻。 2.如何解冻血清才不会
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Amnis公司的ImageStreamX图像流式细胞仪
相关专题 实验室的CT-流式细胞仪Amnis公司的ImageStream图像流式细胞仪 大家应该还记忆犹新吧,它被评为2008年度生命科学十大创新产品之一,是流